Our understanding of cancer genomics is dramatically changing cancer diagnostics, leading the way to personalized cancer care. Delivering comprehensive genomic profiling for clinical cancer care is transforming cancer care. Therapy targeting the alterations that drive an individual patient’s cancer can yield dramatic results. When correctly implemented, next generation sequencing (NGS) methods provide millions of individual observations that are representative of the genetic makeup of a sample. Hybridization capture of target genomic regions allows NGS to be used for comprehensive profiling of clinical oncology specimens. This enduring activity intends to close knowledge and competence gaps regarding the use of comprehensive genomic profiling, allowing physicians to prescribe the most effective, targeted cancer treatments to patients based on the genomic information specific to their own cancer (precision medicine).