New Assay May Help Improve Detection of Prion Diseases

This article originally appeared here.
Share this content:
New Assay May Help Improve Detection of Prion Diseases
New Assay May Help Improve Detection of Prion Diseases

WEDNESDAY, April 13, 2016 (HealthDay News) -- A timed in vitro prion protein (PrP) conversion protocol is sensitive for detecting elk chronic wasting disease (CWD) in brain tissues, according to research published online April 8 in the Journal of Molecular Diagnostics.

As a novel technique for identification of prion disease, John G. Gray, from the Canadian Food Inspection Agency in Lethbridge, and colleagues present a timed in vitro PrP conversion protocol with accurate, well-defined analytical criteria based on probability density and mass functions of transmissible spongiform encephalopathy (TSE)+ and TSE associated thioflavin T signal times. The method was used in elk CWD in brain tissues.

The researchers found that the sensitivity of the protocol and analytical criteria were similar to those of two bioassay models, and more than 1.2 log10 more sensitive than the most sensitive rapid test for TSE. Timing in vitro PrP conversion could be used to titrate TSE infectivity.

"Our results further support that timing in vitro PrP conversion is a plausible method to titrate TSE infectivity," the authors write. "This study embodies the first comprehensive analytical sensitivity comparison between an amyloid seeding assay, bioassay, and multiple TSE rapid tests, thus providing considerable insight into the operating range relation between these TSE detection methods."

The study was supported by the Alberta Livestock and Meat Agency.

Abstract
Full Text (subscription or payment may be required)

Share this content:

is free, fast, and customized just for you!

Already a member?

Sign In Now »

Drug Lookup

Browse drugs by: BrandGenericDisease

Trending Activities

All Professions



Sign up for myCME e-newsletters


More in Home

Pharmacists Should Counsel Patients Fasting for Ramadan

Pharmacists Should Counsel Patients Fasting for Ramadan

Pharmacists can suggest adjustments for meds taken several times per day, those affected by food intake

AUA: Many Have Unused Opioids After Urologic Procedures

AUA: Many Have Unused Opioids After Urologic Procedures

Patients use just over half of initial prescription; highest percentage of unused meds for cystectomy

Over Half of Young Adult Smoke Volume Exposure From Hookahs

Over Half of Young Adult Smoke Volume Exposure ...

Toxicant exposure to tar, carbon monoxide, nicotine lower, but still substantial, compared to cigarettes

is free, fast, and customized just for you!

Already a member?

Sign In Now »