Multi-Layered Microfluidic Device Developed as Model Kidney

This article originally appeared here.
Share this content:
Multi-Layered Microfluidic Device Developed as Model Kidney
Multi-Layered Microfluidic Device Developed as Model Kidney

THURSDAY, Feb. 16, 2017 (HealthDay News) -- A multi-layered microfluidic device has been developed, which closely approximates the in vivo kidney environment, according to research published online Feb. 11 in RSC Advances.

Courtney M. Sakolish, Ph.D., and Gretchen J. Mahler, Ph.D., from Binghamton University in New York, developed a re-useable multi-layered microfluidic device to model the human kidney. The device included a porous growth substrate, physiological fluid flow, and the passive filtration of the glomerulus. HK-2 immortalized human kidney proximal tubule cells were used as the target cell line. The authors exposed cells to a shear stress of 0.8 dyne cm−2 and monitored the cells for protein expression, cytoskeletal reorganization, and increased molecular transport. To allow for more realistic "primary urine" within these devices, an endothelial cell-seeded glomerular filter was used.

The researchers found cells grown within this device exhibited more in vivo-like behavior than cells grown using traditional culturing methods. Glomerular filtration of serum proteins was necessary for healthy cell function. The first in vitro model of passive glomerular filtration coupled within the proximal tubule was provided by the addition of the glomerulus-mimic to the device.

"The multi-layered microfluidic device proposed in this work allows for a close approximation of the in vivo environment that proximal tubule cells flourish in," the authors write. "It has been observed that cells undergo increased junctional formation, polarization, cytoskeletal reorganization, and a significant up-regulation in transport proteins within this device."

Abstract
Full Text

Share this content:

is free, fast, and customized just for you!

Already a member?

Sign In Now »


Sign up for myCME e-newsletters


Drug Lookup

Browse drugs by: BrandGenericDisease

More in Home

ASA: Male Stroke Patients Twice As Likely to Get Timely tPA

ASA: Male Stroke Patients Twice As Likely to ...

Researchers not sure why the disparity exists

CDC: Fatal Drug Overdoses More Than Doubled Since 1999

CDC: Fatal Drug Overdoses More Than Doubled Since ...

Whites, middle-aged adults hardest hit, researchers find

Rates of Resistant Infections Up in U.S. Children

Rates of Resistant Infections Up in U.S. Children

Research highlights increasing community vulnerability

is free, fast, and customized just for you!

Already a member?

Sign In Now »