'Expansion Pathology' Method Could Mean Earlier Intervention

This article originally appeared here.
Share this content:
'Expansion Pathology' Method Could Mean Earlier Intervention
'Expansion Pathology' Method Could Mean Earlier Intervention

FRIDAY, July 21, 2017 (HealthDay News) -- A new method, called expansion pathology (ExPath), which is a clinically optimized form of expansion microscopy (ExM), can be used for pathology and clinical research, according to a report published online July 17 in Nature Biotechnology.

Yongxin Zhao, Ph.D., from the Massachusetts Institute of Technology in Cambridge, and colleagues describe a clinically optimized form of ExM that supports nanoscale imaging of human tissue specimens that have been fixed with formalin, embedded in paraffin, stained with hematoxylin and eosin, and/or fresh frozen. The method converts clinical samples into an ExM-compatible state, then applies a clinical sample-optimized ExM protocol with protein anchoring and mechanical homogenization.

The researchers found that using conventional diffraction-limited microscopes and standard antibody and fluorescent DNA in situ hybridization reagents, ExPath allows about 70-nm-resolution imaging of diverse biomolecules in intact tissues. ExPath was used for optical diagnosis of kidney minimal-change disease, which previously required electron microscopy. In addition, ExPath showed high-fidelity for computational discrimination between early breast neoplastic lesions, for which pathologists often disagree.

"ExPath may enable the routine use of nanoscale imaging in pathology and clinical research," the authors write.

Abstract
Full Text (subscription or payment may be required)

Share this content:

is free, fast, and customized just for you!

Already a member?

Sign In Now »


Sign up for myCME e-newsletters


Drug Lookup

Browse drugs by: BrandGenericDisease

Trending Activities

All Professions

More in Home

FDA Approves New Treatment for Acute Lymphoblastic Leukemia

FDA Approves New Treatment for Acute Lymphoblastic Leukemia

For patients with relapsed or refractory B-cell ALL who have received one or two prior treatments

Online Nursing Education Can Up Patient Use of VTE Prophylaxis

Online Nursing Education Can Up Patient Use of ...

Online training aims to make sure those hospitalized get treatment to prevent venous thromboembolism

On-Pump CABG Leads to Higher Rates of Five-Year Survival

On-Pump CABG Leads to Higher Rates of Five-Year ...

Study compared coronary-artery bypass grafting surgery, with/without cardiopulmonary bypass

is free, fast, and customized just for you!

Already a member?

Sign In Now »